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KMID : 1101320050370030139
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Korean Journal of Clinical Laboratory Science
2005 Volume.37 No. 3 p.139 ~ p.142
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Genotyping of HLA-DRB1 by Polymerase Chain Reaction-Sequence Specific Primer
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Jang Soon-Mo
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Abstract
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Most expressed HLA(human leukocyte antigen) loci exhibit a remarkable degree of allelic polymorphism, which is derived from sequenceing differences predominantly localized to discrete hypervariable regions of the amino-terminal domain of the molecule. In this study, the HLA-DRB1 genotypes were determined in twenty students using the PCR-SSP (polymerase chain reaction-sequence specific primer) technique. Two specific primer pairs in assigning the DRB1 gene were used. The results of PCR-SSP, the HLA-DRB1*0101 primer detected nine and HLA-DRB1*1501 primer detected three people. This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-DRB1 genotypes. Moreover, these genotype frequency results of the HLA DRB1 gene could be useful for database study before being applied to individual identification and transplantation immunity.
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KEYWORD
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HLA, HLA-DRB1, PCR-SSP
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